Massive RT-PCR Primer Designer is online

gaoch

2016-01-16

Categories: 其它 信息技术 生物学 Tags: 旧文 WordPress BioPerl primer design RT-PCR

The Massive RT-PCR Primer Designer is based on Primer3 and BioPerl toolbox, and was derived from the standalone codes which have been discussed by two previous blog posts (Post 1 and Post 2).

rt-primer-designer

You may access the service at The Massive RT-PCR Primer Designer.

What this tool needs is your fasta file, which contains one or more nucleotide sequences that will be use as PCR template. Upload this file and the tool will generate the most ideal primer pairs suitable for quantitive Real-Time PCR (qRT-PCR). The primers will be supplied as a downloadable Excel file.

Notably, the amplicon size of these RT-PCR primers are spanning from 150 bp to 250 bp.

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作者简介

Chun-Hui Gao is a Research Associate at Huazhong Agricultural University.

重复使用

Text and figures are licensed under Creative Commons Attribution CC BY 4.0. The source code is licensed under MIT. The full source is available at https://github.com/yihui/hugo-prose.

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gaoch (2016). Massive RT-PCR Primer Designer is online. BIO-SPRING. /post/2016/01/16/2016-01-16-massive-rt-pcr-primer-designer-is-online/

BibTeX citation

@misc{
  title = "Massive RT-PCR Primer Designer is online",
  author = "gaoch",
  year = "2016",
  journal = "BIO-SPRING",
  note = "/post/2016/01/16/2016-01-16-massive-rt-pcr-primer-designer-is-online/"
}